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The production of 6‐aminopenicillanic acid by a multistage tubular reactor packed with immobilized penicillin amidase
Author(s) -
Park Jong M.,
Choi Cha Y.,
Seong Baik L.,
Han Moon H.
Publication year - 1982
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.260240714
Subject(s) - penicillin amidase , packed bed , chemistry , chromatography , immobilized enzyme , plug flow reactor model , plug flow , drop (telecommunication) , enzyme , continuous stirred tank reactor , thermodynamics , biochemistry , physics , telecommunications , computer science
A theoretical model equation was derived to find the correlation between the conversion and the amount of immobilized penicillin amidase in column. The theoretical values of the conversion were predicted form this correlation and compared with experimental results. It was observed in a column reactor that the pH drop along the column path was linear versus the enzyme loading and that the enzyme activity was also linearly dependent on pH up to 8.0. In order to diminish the effect of pH drop, a continuous two‐stage plug‐flow reactor (PFR) with pH adjustment between the two columns was used was used in the experiments, and two‐ and three‐stage PFRs were simulated by computer. In the case of the two‐stage PFR, the maximum productivity was demonstrated experimentally and theoretically as well. when an equal amount of the immobilized enzyme was packed in both columns. It was also predicted in the tree‐stage PFR system that the optimal distributions of enzyme loading in three columns were found to be 1:1:1. It was demonstrated that the increased number of reactors in series could enhance the level of the maximum productivity with a given amount of enzyme loading.