Premium
A Computer‐aided noninterfering on‐line technique for monitoring oxygen‐transfer characteristics during fermentation processes
Author(s) -
Spriet J. A.,
Botterman J.,
Buyser D. R. De,
De Visscher P. L.,
Vandamme E. J.
Publication year - 1982
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.260240713
Subject(s) - process engineering , process (computing) , sensitivity (control systems) , fermentation , line (geometry) , biological system , computer science , operator (biology) , biochemical engineering , mathematics , chemistry , engineering , electronic engineering , biochemistry , geometry , food science , repressor , transcription factor , gene , biology , operating system
The role of computers in the monitoring and control of fermentation processes has increased steadfastly. The ultimate utility of the machines will not depend on the availability of online sensors but also on the availability of techniques that combine direct measurements, leading towards estimates of variable closely related to the microbial process or its control. In this article, a methodology for on‐line and noninterfering evaluation of the volumetric mass‐transfer coefficient K l a is developed. A detailed presentation of the procedure, called “the static method,” is given. Its feasibility is proved through implementation of the method on an antibiotic fermentation process. These experiments indicate that operator actions meant to modify the oxygen‐transfer conditions can be checked on‐line. The quantitative value of the static method is ascertained by comparing the experimental results with K l a estimates obtained with the “gassing‐out” method. A sensitivity analysis was carried out, revealing the need for temperature and pressure corrections and showing that the precision of the oxygen analyzer determines the precision of the static method.