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Modeling enzyme immobilization in porous solid supports
Author(s) -
Do Duong D.,
Clark Douglas S.,
Bailey James E.
Publication year - 1982
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.260240707
Subject(s) - immobilized enzyme , enzyme , porosity , chemical engineering , chemistry , particle (ecology) , mass transfer , enzyme assay , chromatography , catalysis , process (computing) , kinetics , materials science , organic chemistry , computer science , oceanography , engineering , geology , operating system , physics , quantum mechanics
Enzymes are often immobilized on the internal surfaces of porous solid by immersing enzyme‐free particles in a well mixed solution of enzyme. The ensuing impregnation process involves coupled transient mass transfer and surface attachment of enzyme. A mathematical model is employed to explore the influences of process parameters on the amount of enzyme loaded and the distribution of immobilized enzyme within the support particles. Nonuniform loading of the support occurs under some conditions. This is significant since the distribution of enzyme within the support particle influences the overall activity and stability of the immobilized enzyme catalyst. The model developed here may also be used to describe removal of reversibly immobilized enzyme during washing or utilization of the immobilized enzyme catalyst.

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