Premium
Studies on the characteristics of alginate gels in relation to their use in separation and immobilized applications
Author(s) -
Kierstan M.,
Darcy G.,
Reilly J.
Publication year - 1982
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.260240705
Subject(s) - diffusion , calcium alginate , porosity , chromatography , chemistry , chemical engineering , hemoglobin , nad+ kinase , calcium , biochemistry , thermodynamics , organic chemistry , enzyme , engineering , physics
Studies on diffusion of NAD and hemoglobin from calcium and barium gels are reported where alginate grade, concentration, and gel dimensions were varied. These show that NAD diffusion characteristics are unaffected by alginate and ion concentrations; however, hemoglobin diffusion is affected by alginate concentration. Both hemoglobin and NAD diffusion patterns were shown to be affected by alginate gel dimensions. Studies are reported that show that polymannuronic alginate gels posses good porosity characteristics while polyguluronic alginates from gels with lower porosity, specifically with respect to high‐molecular‐weight compounds. These findings are discussed with the view to the use of alginate gels for immobilization, solids separation, and diffusion chromatography techniques.