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Continuous ethanol production from Jerusalem artichoke tubers. II. Use of immobilized cells of Kluyveromyces marxianus
Author(s) -
Margaritis Argyrios,
Bajpai Pratima
Publication year - 1982
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.260240703
Subject(s) - kluyveromyces marxianus , jerusalem artichoke , bioreactor , ethanol fuel , ethanol , chemistry , biomass (ecology) , chromatography , sugar , biochemistry , yeast , food science , botany , biology , saccharomyces cerevisiae , organic chemistry , agronomy
Kluyveromyces marxianus UCD (FST) 55–82 cells were immobilized in Na alginate beads and used in a packed‐bed bioreactor system for the continuous production of ethanol from the extract of Jerusalem artichoke tubers. Volumetric ethanol productivities of 104 and 80 g ethanol/ L/h were obtained at 80 and 92% sugar utilization, respectively. The maximum volumetric ethanol productivity of the immobilized cell bioreactor system was found to be 15 times higher than that of an ordinary‐stirred‐tank (CST) bioreactor using cells of K. marxianus . The immobilized cell bioreactor system was operated continuously at a constant dilution rate of 0.66 h −1 for 12 days resulting in only an 8% loss of the original immobilized cell activity, which corresponds to an estimated half‐life of ca. 72 days. The maximum specific ethanol productivity and maximum specific sugar uptake rate of the immobilized cells were found to be 0.55 g ethanol/g/biomass/h and 1.21 g sugars/g biomass/h, respectively.