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Enzymatic hydrolysis of cellulose and various pretreated wood fractions
Author(s) -
Saddler J. N.,
Brownell H. H.,
Clermont L. P.,
Levitin N.
Publication year - 1982
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.260240611
Subject(s) - trichoderma reesei , cellulose , enzymatic hydrolysis , hydrolysis , chemistry , lignin , cellulosic ethanol , cellulase , steam explosion , substrate (aquarium) , chromatography , organic chemistry , pulp and paper industry , biology , ecology , engineering
Three strains of Trichoderma — T. reesei C30, T. reesei QM9414, and Trichoderma species E‐58—were used to study the enzymatic hydrolysis of pretreated wood substrates. ach of the culture filtrates was incubated with a variety of commercially prepared cellulose substrates and pretreated wood substrates. Solka floc was the most easily degraded commercial cellulose. The enzyme accessibility of steam‐exploded samples which had been alkali extracted and then stored wet decreased with the duration of the steam treatment. Air drying reduced the extent of hydrolysis of all the samples but had a greater effect on the samples which had previously shown the greatest hydrolysis. Mild pulping using 2% chlorite increased the enzymatic hydrolysis of all the samples. Steam explosion was shown to be an excellent pretreatment. The results indicate that the distribution of the lignin as well as the surface area of the cellulosic substrate are important features in enzymatic hydrolysis.