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Quantitative measurement of oxygen consumption in insect cell culture infected with polyhedrosis virus
Author(s) -
Weiss S. A.,
Orr T.,
Smith G. C.,
Kalter S. S.,
Vaughn J. L.,
Dougherty E. M.
Publication year - 1982
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.260240511
Subject(s) - cell culture , fermentation , biology , baculoviridae , oxygen , virus , insect , nuclear polyhedrosis virus , microbiology and biotechnology , virology , chemistry , biochemistry , botany , genetics , organic chemistry , spodoptera , gene , recombinant dna
A simple and reproducible quantitative method for measuring dissolved oxygen (DO) in uninfected and baculovirus infected cells in culture is described. To establish this method, an industrial DO measuring system for fermentation was employed. During this process the physical characteristics of the cell culture vessel were taken into account permitting a direct readout of DO in microliters per vessel. During these studies, it was experimentally documented that insect cells, particularly baculovirus infected cells, in culture from 1 to 14 days utilize an appreciable level of DO.