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Potentiometric quantitation of glycerol using immobilized glycerol dehydrogenase
Author(s) -
Chen A. K.,
Starzmann J. A.,
Liu C. C.
Publication year - 1982
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.260240416
Subject(s) - glycerol , chemistry , dehydrogenase , chromatography , potentiometric titration , glycerol kinase , biochemistry , enzyme , organic chemistry , ion
Glycerol dehydrogenase was immobilized in polyacrylamide gel layered over a small platinum screen and used to catalyze the oxidation of glycerol. In the presence of NAD + and potassium ferricyanide, the coupling reaction generated a measurable electrical potential which was found to be Nernstian with respect to the glycerol concentration range of 10 −4 M to 10 −1 M . The reproducibility of the measurement and the optimal conditions for glycerol determination were described.