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A kinetic expression for hydrolysis of soluble starch by glucoamylase
Author(s) -
Kusunoki Koichiro,
Kawakami Koei,
Shiraishi Fumihide,
Kato Keiichi,
Kai Mayumi
Publication year - 1982
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.260240208
Subject(s) - hydrolysis , starch , chemistry , kinetic energy , biochemistry , chemical engineering , chromatography , physics , quantum mechanics , engineering
As the hydrolysis of starch by glucoamylase proceeds with stepwise removal of glucose units from the nonreducing ends of the starch chain, the number of available substrate molecules is essentially unchanged in the course of the degradation. In view of this aspect, a simple practical kinetic expression, which consists of a modified Michaelis–Menten form with product inhibition, is presented for the hydrolysis of soluble starch. It is assumed that the values of kinetic parameters V m and K m vary linearly from the values for starch toward those for maltose. The applicability of this kinetic expression is verified through the simulation with the experimental results for the hydrolysis of two soluble starches with different average molecular weights of 3 × 10 4 and 3 × 10 6 .