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Mutagenesis and carcinogenesis testing in vitro
Author(s) -
Casto Bruce C.
Publication year - 1981
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.260231203
Subject(s) - carcinogenesis , in vivo , mutagenesis , computational biology , in vitro , predictability , biology , carcinogen , biochemical engineering , cancer , bioinformatics , microbiology and biotechnology , biochemistry , genetics , mutation , gene , mathematics , engineering , statistics
The development and validation of short‐term tests for carcinogens and mutagens are urgently needed if estimates of 70–80% for environmentally induced cancer are valid. Several methods for in vitro testing have been described in microbial and mammalian cells. It is anticipated that a battery of tests will soon be available with high degree of reproducibility and predictability for potential in vivo carcinogenesis. This review discusses those assays currently being implemented in mammalian cells including a brief description of the methodology, advantages, and disadvantages, and the correlation with known in vivo activity.