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Kinetic modeling of a multiple immobilized enzyme system. I. Development and testing of the model
Author(s) -
Mercer D. G.,
O'Driscoll K. F.
Publication year - 1981
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.260231106
Subject(s) - effluent , bioreactor , sucrose , immobilized enzyme , invertase , substrate (aquarium) , glucose oxidase , chemistry , packed bed , chromatography , chemical engineering , enzyme , biochemistry , organic chemistry , environmental science , environmental engineering , biology , engineering , ecology
A kinetic model for the reaction sequence catalyzed by coimmobilized invertase and glucose oxidase with a sucrose substrate in a tubular reactor has been developed. The computerized mathematical model employs and orthogonal collection technique for solving oxidase were coimmobilized in poly(2‐hydroxyethlmethacrylate) gels and used in a continuous flow packed‐bed tubular reactor system. In addition to describing the development of the kinetic model, this article compares experimentally determined reactor effluent concentrations for various sucrose feed solutions to those predicted by the model. Variations between experimental and predicted reactor effluent concentrations were found to be on the micromolar level for sucrose feed concentrations as low as 1.38m M .