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Photochemical energy conversion system using immobilized chloroplasts
Author(s) -
Kayano Hiromichi,
Matsunga Tadashi,
Karube Isao,
Suzuki Shuichi
Publication year - 1981
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.260231011
Subject(s) - photocurrent , clostridium butyricum , chloroplast , photochemistry , chemistry , ferredoxin , hydrogen , materials science , organic chemistry , biochemistry , optoelectronics , fermentation , gene , enzyme
Immobilized chloroplasts and Clostridium butyricum were employed for a photochemical energy conversion system. Spinach chloroplasts were immobilized in 2% agar gel. The optimum temperature of immobilized chloroplasts was 30°C. The maximum activity was obtained in the phosphate buffer solution (pH 8.0) containing 8μ M of ferredoxin under an N 2 bubbling condition. Hydrogen was evolved under illumination by immobilized chloroplasts and C. butyricum . Hydrogen produced by this system was applied to a hydrogen‐oxygen fuel cell. Photoinduced current was obtained from this photochemical energy conversion system. A photocurrent of 0.4−1.5 mA was continuously obtained for 4 h. The conversion ratio from hydrogen to current was 80−100%.