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A CSTR‐hollow fiber system for continuous hydrolysis of proteins. Performance and kinetics
Author(s) -
Deeslie W. David,
Cheryan Munir
Publication year - 1981
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.260231009
Subject(s) - continuous stirred tank reactor , chemistry , residence time distribution , residence time (fluid dynamics) , kinetics , batch reactor , chromatography , membrane reactor , plug flow reactor model , substrate (aquarium) , fiber , thermodynamics , membrane , biochemistry , organic chemistry , mineralogy , catalysis , biology , inclusion (mineral) , ecology , physics , geotechnical engineering , quantum mechanics , engineering
The effect of four operating variables (enzyme concentration, substrate concentration, flow rate, and reaction volume) on the performance of CSTR‐hollow fiber membrane reactor was studied for the continuous hydrolysis of a soy protein isolate using Pronase. Based on a residence time distribution study, the reactor system was modeled as an ideal CSTR in combination with the Michaelis‐Menten equation of enzyme kinetics. This kinetic model correlated conversion with a space‐time parameter modified to include all four independent variables. An empirical model based on curvilinear regression analysis was also developed. Both models predicted conversion fairly well, although the kinetic model slightly underpredicts at high conversion.