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Continuous cultivation of fission yeast: Classical and flow microfluorometry observations
Author(s) -
Agar David W.,
Bailey James E.
Publication year - 1981
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.260231006
Subject(s) - schizosaccharomyces pombe , yeast , dilution , steady state (chemistry) , schizosaccharomyces , fission , chemistry , dna , biophysics , saccharomyces cerevisiae , biology , biochemistry , thermodynamics , physics , quantum mechanics , neutron
The fission yeast Schizosaccharomyces pombe was grown in glucose‐limited medium in a steady‐state continuous flow reactor. Changes in mean cell protein and RNA contents with growth rate are consistent with earlier observations under different conditions. Flow microfluorometry measurements of the frequency functions of DNA at different dilution rates show changes in coordination of DNA synthesis and cell separation. Shifting from batch growth to small dilution rates results in unusual cell aggregation which leads to multiple steady states at identical operating conditions.