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Pulse response in an immobilized‐enzyme column: Elution profiles in reversible and consecutive reactions
Author(s) -
Adachi Shuji,
Hashimoto Kenji,
Miyai Kiyosato,
Kurome Hirokazu,
Matsuno Ryuichi,
Kamikubo Tadashi
Publication year - 1981
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.260230903
Subject(s) - elution , chemistry , substrate (aquarium) , chromatography , glucose oxidase , invertase , immobilized enzyme , enzyme , pulse (music) , isomerase , michaelis–menten kinetics , enzyme assay , biochemistry , oceanography , engineering , detector , electrical engineering , geology
A pulse of substrate solution was applied to an immobilized‐enzyme column, in which the substrate was then converted by reversible or consecutive reactions. Immobilized glucose isomerase was used for the reversible reaction, and immobilized invertase and glucose oxidase for the consecutive reactions. The elution profiles of substrate and product were determined experimentally. These profiles were in good agreement with the ones predicted theoretically. The effect of some parameters on the elution profiles for reversible and consecutive reactions is discussed.