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Fed‐batch culture: Modeling and application in the study of microbiol energetics
Author(s) -
Esener A. A.,
Roels J. A.,
Kossen N. W. F.
Publication year - 1981
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.260230812
Subject(s) - energetics , klebsiella pneumoniae , batch processing , kinetics , bacterial growth , biological system , transient (computer programming) , bacteria , chemistry , biology , computer science , biochemistry , ecology , physics , escherichia coli , genetics , quantum mechanics , gene , programming language , operating system
Microbial growth in the fed‐batch mode is described by a simple unstructured model. The model is found to be in good agreement with agreement with the experimental observation, except under highly transient conditions. Extensive experimental data were collected and the energetics of the bacterium Klebsiella pneumoniae is evaluated. It is shown that the fed‐batch culti vation is a powerful experimental tool in the study of microbial kinetics and energetics simultaneously. Methods for determining the maintenance requirements are shown and evaluated. The maintenance coefficients determined from fed‐batch data are systematically smaller than those reported for continuous culture systems. Results suggest a decrease in maintenance demands at low specific growth rates.