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Hydrolysis of triglyceride by solid phase lipolytic enzymes of Rhizopus arrhizus in continuous reactor systems
Author(s) -
Bell George,
Todd John R.,
Blain John A.,
Patterson John D. E.,
Shaw Charles E. L.
Publication year - 1981
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.260230804
Subject(s) - rhizopus arrhizus , hydrolysis , chemistry , chromatography , continuous stirred tank reactor , yield (engineering) , interesterified fat , lipase , aqueous solution , immobilized enzyme , triglyceride , batch reactor , aqueous two phase system , organic chemistry , enzyme , materials science , biochemistry , catalysis , cholesterol , metallurgy
Continuous hydrolysis of triglyceride in organic solvent systems using Rhizopus arrhizus mycelia as a source of insolubilized lipase has been studied in packed‐bed and stirred‐tank reactors. Typically a packed bed reactor containing 1 g of mycelia fed at 1 mL/min with a solution of 2.5% (w/v) olive oil in di‐isopropyl ether gave a fatty acid yield of 45% at 30°C. The optimum water concentration was found to be 0.17% (w/v) except under conditions of high oil feed concentration and high yield where no optimum was established. No temperature optimum was observed over the range 20–55°C. Calculated activation energies of 13–20 kJ/mol, depending on temperature, were lower, while K m (app) values of 0.1–0.3 M were higher than those for hydrolysis in conventional aqueous emulsion systems. No evidence of any significant diffusional limitation, which could account for these values, was obtained. The mycelia showed a loss of activity of 0.6–1.0%h at 30°C. The packed bed proved markedly superior to the stirred tank for this system.