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Automatic control for fed‐batch culture of single cell protein
Author(s) -
Huang Shih Yow,
Chu WeyBang
Publication year - 1981
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.260230709
Subject(s) - ethanol , oxygen , liter , fermentation , fed batch culture , chemistry , signal (programming language) , ethanol fuel , ethanol content , chromatography , pulp and paper industry , analytical chemistry (journal) , biochemistry , biology , computer science , organic chemistry , engineering , programming language , endocrinology
Candida utilis was cultivated in a 5‐liter jar fermentor using ethanol as sole carbon source. Control of ethanol in the cultivation broth was performed by using an ethanol vapor monitoring instrument and an oxygen electrode coupled with two control circuits. By setting upper and lower bounds according to the predetermined conditions, a signal from a gas monitoring sensor switched the lower or higher bound relay governing the actuating or switch‐off of the motor; this maintained a proper concentration of ethanol in the cultivation of ethanol in the system. The growth of cells was found to be satisfactory. Cell concentration reached 64 g/liter during a 20‐hr cultivation. As the results of comparative experiments, the control mode using the gas monitoring instrument was found to be superior to that using dissolved oxygen as a controlling signal, especially at high cell concentration.