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Use of glucose oxidase system in measuring aeration capacity of fermentors. Comparison of the dynamic and steady‐state methods of k l a measurement
Author(s) -
Linek V.,
Beneš P.,
Hovorka F.,
Holeček O.
Publication year - 1981
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.260230707
Subject(s) - aeration , glucose oxidase , steady state (chemistry) , chemistry , substrate (aquarium) , kinetics , michaelis–menten kinetics , oxygen , chromatography , hydrolysis , thermodynamics , analytical chemistry (journal) , enzyme , enzyme assay , biochemistry , organic chemistry , oceanography , physics , quantum mechanics , geology
A steady‐state method for k l a determination has been presented using the Michaelis–Menten two‐substrate kinetic equation for oxidation of glucose in the presence of the enzymes glucose oxidase and excess catalase. The conditions have been specified where spontaneous hydrolysis of lactone is sufficiently rapid, thus eliminating inhibitory action of lactone on the oxidation. In glucose oxidase‐free batches, the k l a values were determined using various modification of the dynamic method. The dynamic methods in which gas interchange was effected without interrupting aeration and agitation of the batch yielded erroneously lower k l a values as compared to the results of steady‐state methods if the measured k l a value was higher than 0.03 s −1 . The values yielded by the dynamic method in which the gas interchange was effected at the same time with turning on aeration and agitation of the batch agreed with values resulting from the steady‐state method provided that the measured k l a values were lower than 0.08 −1 and the simultaneous interfacial transport of nitrogen and oxygen had been taken into account in the evaluation. At k l a values higher than 0.08 −1 , this modification of the dynamic method also yielded lower k l a values as compared with the outcome of the steady‐state method. The experiments performed do not, however, allow one to decide unambiguously on the whether these lower k l a values are due to failure of the adopted model to describe adequately the dynamic behavior of the system or whether they are true values differing from those yielded by the steady‐state method on account of different physical properties of compared batches.