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Kinetics of the isomerization of D ‐glucose into D ‐fructose catalyzed by glucose isomerase containing Arthrobacter cells in immobilized and nonimmobilized form
Author(s) -
Van Keulen M. A.,
Vellenga K.,
Joosten G. E. H.
Publication year - 1981
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.260230704
Subject(s) - kinetics , isomerase , isomerization , chemistry , glucose 6 phosphate isomerase , fructose , catalysis , biochemistry , membrane , toluene , permeability (electromagnetism) , chromatography , enzyme , organic chemistry , physics , quantum mechanics
The kinetic properties of immobilized and nonimmobilized glucose isomerase have been investigated. In both cases the kinetics can be described by a modified Michaelis–Menten expression. It appeared that immobilization causes no deactivation. Furthermore, it was shown that the permeability of the cell membrane increases by heat and toluene treatments.