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The isolation of lytic enzymes from Cytophaga and their application to the rupture of yeast cells
Author(s) -
Asenjo J. A.,
Dunnill P.
Publication year - 1981
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.260230512
Subject(s) - lytic cycle , cytophaga , yeast , enzyme , isolation (microbiology) , biochemistry , chemistry , chromatography , precipitation , hydrolysis , microbiology and biotechnology , biology , bacteria , pseudomonas , virology , virus , genetics , flavobacterium , physics , meteorology
Yeast–lytic enzymes have been isolated on a pilot scale from Cytophaga species by precipitation and the light, enzyme‐rich solid phase recovered by liquid‐liquid separation. The enzyme complex was immobilized to soluble polymeric carbohydrates and the effectiveness of the free and immobilized enzyme for protein release and cell debris dissolution has been assessed.