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Enhancement of oxygen absorption by magnetite‐containing beads of immobilized glucose oxidase
Author(s) -
Sada Eizo,
Katoh Shigeo,
Terashima Masaaki
Publication year - 1981
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.260230511
Subject(s) - glucose oxidase , magnetite , absorption (acoustics) , chemistry , oxygen , mass transfer , chromatography , immobilized enzyme , limiting oxygen concentration , chemical engineering , biosensor , analytical chemistry (journal) , materials science , organic chemistry , enzyme , biochemistry , composite material , metallurgy , engineering
Rates of oxygen absorption into glucose solutions were measured using an immobilized‐enzyme reactor, in which magnetite‐containing beads of immobilized glucose oxidase were moved by a revolving magnetic field to reduce the mass transfer resistances at the gas–liquid interface and around the bead. Data were also obtained for oxygen absorption into glucose solutions containing soluble or immobilized glucose oxidase (without magnetite), as well as for physical absorption of oxygen. The rates of physical absorption for the runs with the magnetite‐containing beads increased because of mechanical stirring caused by spinning of the beads at the gas‐liquid interface. In this case the experimental enhancement factors were found to be larger than those predicted on the basis of the film theory for gas absorption with a pseudo‐first order reaction.