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Stabilization of chloroplast by radiation‐induced immobilization with various glass‐forming monomers
Author(s) -
Youshii F.,
Fujimura T.,
Kaetsu I.
Publication year - 1981
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.260230413
Subject(s) - monomer , thermostability , chloroplast , polymerization , chemistry , polymer chemistry , chemical engineering , polymer , organic chemistry , biochemistry , engineering , gene , enzyme
The effect of immobilization with various glass‐forming monomers on the stability of PS II activity of spinach chloroplast was investigated. PS II activity (O 2 evolution due to the Hill reaction) was reduced very slightly by the addition of monomers including polyethyleneglycol (PEG). Immobilization of chloroplast was done with hydrophobic monomer as well as hydrophilic monomer and activity of immobilized chloroplast increased with decreasing monomer concentration as far as the polymerization was possible. The activity of immobilized chloroplast was very high and it decayed far more gradually with the storage time in comparison with the decay of unimmobilized chloroplast and was retained more than 30 days. The optimum monomer concentration for immobilization was about 10%. Thermostability of chloroplast also increased greatly by immobilization with these monomers, especially hydrophilic monomers.