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Casein hydrolysis in stirred tank reactors using chymotrypsin immobilized on magnetic supports
Author(s) -
Munro P. A.,
Dunnil P.,
Lilly M. D.
Publication year - 1981
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.260230403
Subject(s) - hydrolysis , continuous stirred tank reactor , batch reactor , nickel , magnetic separation , separator (oil production) , chromatography , bioreactor , chemical engineering , chemistry , catalysis , casein , materials science , organic chemistry , metallurgy , thermodynamics , physics , engineering
Nickel‐NiO‐BSA‐chymotrypsin has been used to hydrolyze casein in both batch and continuous stirred tank reactors. The kinetics of hydrolysis in both types of reactor were considered. Some operational problems encountered using fine powder catalysts in batch stirred tank reactors are discussed. High gradient magnetic separation was found to be a powerful catalysts retention method for magnetic support particles, particularly when using a ferromagnetic collection matrix. Nickel particles of diameter 3–7 μm were easily separated from water at a processing velocity of 39 mm/sec. Nickel powder and precipitated Fe 3 O 4 were also separated satisfactorily using a rotating drum magnetic separator.