Premium
Application of microbial cells as multistep catalysts in potentiometric biosensing electrodes
Author(s) -
Kobos R. K.,
Pyon H. Y.
Publication year - 1981
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.260230312
Subject(s) - nitrilotriacetic acid , potentiometric titration , biosensor , electrode , chemistry , ammonia , inorganic chemistry , biochemistry , chelation
The advantages of using intact microbial cells to mediate complex reaction sequences in the construction of biosensing electrodes are domonstrated. A potentiometric sensor was developed for nitrilotriacetic acid (NTA) in which a strain of Pseudomonas sp. was coupled to an ammonia gas‐sensing electrode. the bacterial cells carry out a four‐step reaction sequence to produce the measured species, ammonia, from NTA. The resulting microbial electrode responded to NTA in the concentration range of 1 × 10 −4 M to 7 × 10 −4 M , with a slope of 35‐40 mV/decade. The electrode was stable for up to one month.