Peptide synthesis enzymatically catalyzed in a biphasic system: Water– water–immiscible  organic solvent | Zendy

Semenov Anatole N. | Zendy; Berezin Ilya V. | Zendy; Martinek Karel | Zendy

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Peptide synthesis enzymatically catalyzed in a biphasic system: Water– water–immiscible organic solvent

Author(s) -

Semenov Anatole N.,

Berezin Ilya V.,

Martinek Karel

Publication year - 1981

Publication title -

biotechnology and bioengineering

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.136

H-Index - 189

eISSN - 1097-0290

pISSN - 0006-3592

DOI - 10.1002/bit.260230210

Subject(s) - chemistry , yield (engineering) , solvent , reagent , stoichiometry , catalysis , peptide bond , peptide synthesis , amide , organic solvent , chymotrypsin , organic chemistry , peptide , medicinal chemistry , enzyme , trypsin , chemical engineering , biochemistry , materials science , engineering , metallurgy

Synthesis of a peptide bond is suggested to be enzymatically catalysed in a biphasic system “water– water‐immiscible organic solvent”. The pH dependence of the apparent equilibrium constant is studied for synthesis of N ‐acetyl‐ L ‐tryptophanyl‐ L ‐leucine amide from N ‐Acet‐Trp and L ‐Leu‐NH 2 . The reaction was performed in the biphasic system ethyl acetate plus water [from 2 to 2% (v/v)] in the presence of α‐chymotrypsin. The suggested approach is preparative value: with the stoichiometric ratio of the reagents, [ N ‐Acet‐ L ‐Trp] = [ L ‐Leu‐NH 2 ] = 2 × 10 −3 M , the yield is practically 100% (in water, with other conditions being the same, the yield is not over 01.%).

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