Premium
Peptide synthesis enzymatically catalyzed in a biphasic system: Water– water–immiscible organic solvent
Author(s) -
Semenov Anatole N.,
Berezin Ilya V.,
Martinek Karel
Publication year - 1981
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.260230210
Subject(s) - chemistry , yield (engineering) , solvent , reagent , stoichiometry , catalysis , peptide bond , peptide synthesis , amide , organic solvent , chymotrypsin , organic chemistry , peptide , medicinal chemistry , enzyme , trypsin , chemical engineering , biochemistry , materials science , engineering , metallurgy
Abstract Synthesis of a peptide bond is suggested to be enzymatically catalysed in a biphasic system “water– water‐immiscible organic solvent”. The pH dependence of the apparent equilibrium constant is studied for synthesis of N ‐acetyl‐ L ‐tryptophanyl‐ L ‐leucine amide from N ‐Acet‐Trp and L ‐Leu‐NH 2 . The reaction was performed in the biphasic system ethyl acetate plus water [from 2 to 2% (v/v)] in the presence of α‐chymotrypsin. The suggested approach is preparative value: with the stoichiometric ratio of the reagents, [ N ‐Acet‐ L ‐Trp] = [ L ‐Leu‐NH 2 ] = 2 × 10 −3 M , the yield is practically 100% (in water, with other conditions being the same, the yield is not over 01.%).