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An outdoor biophotolytic system using the cyanobacterium Anabaena cylindrica B629
Author(s) -
Smith Geoffrey D.,
Lambert Grant R.
Publication year - 1981
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.260230114
Subject(s) - anabaena , acetylene , nitrogen , sodium bicarbonate , cyanobacteria , oxygen , chemistry , carbon monoxide , bicarbonate , incubation , botany , atmosphere (unit) , environmental chemistry , bacteria , biology , biochemistry , organic chemistry , genetics , physics , catalysis , thermodynamics
The cyanobacterium Anabaena cylindrica B629 was suspended in small glass beds and incubated in a gas‐tight glass vessel outdoors under a gas atmosphere comprising carbon monoxide (0.2%), acetylene (5%), oxygen (6.5%), and nitrogen. The solution phase initially contained sodium bicarbonate (10mM) at pH 7. Under these conditions the organism continuously produced hydrogen gas for over three weeks. The temperature of the culture was maintained below 30°C and minimum night temperatures were recorded. The vessel was covered by a shadecloth, which reduced the natural illumination by approximately 70%. The system is an alternative to those requiring the strict absence of oxygen and little nitrogen, and requires virtually no attention during the incubation period.