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Production of yeast‐lytic enzymes by Cytophaga species in batch culture
Author(s) -
Asenjo J. A.,
Dunnill P.,
Lilly M. D.
Publication year - 1981
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.260230108
Subject(s) - cytophaga , lytic cycle , fermentation , yeast , enzyme , biology , yeast extract , industrial fermentation , chemically defined medium , biochemistry , microbiology and biotechnology , chemistry , food science , bacteria , in vitro , pseudomonas , virus , genetics , flavobacterium , virology
The conditions for culture storage, inoculum preparation, and growth of a Cytophaga species, constitutive with respect to yeast‐lytic enzymes, have been established in shake‐flask studies and in 5 liter fermentor experiments. A low cost medium was adopted for 900 liter‐scale fermentation and gave an enzyme activity in the fermentation broth somewhat greater than the comparable laboratory‐scale one.