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Enzyme activity maintenance in packed‐bed reactors via continuous enzyme addition
Author(s) -
Verhoff F. H.,
Schlager S. T.
Publication year - 1981
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.260230105
Subject(s) - immobilized enzyme , enzyme , chemistry , volumetric flow rate , packed bed , enzyme assay , chromatography , volume (thermodynamics) , constant (computer programming) , bioreactor , mechanics , biochemistry , thermodynamics , computer science , organic chemistry , physics , programming language
An operational scheme for using immobilized enzymes in packed‐bed reactors that permits operation at a constant throughput rate and constant product quality is described. The scheme used columns operated in series with continuous enzyme addition to compensate for enzyme decay. A mathematical technique was developed to determine the enzyme addition rate, enzyme usage, and enzyme volume in the column system. Operation of columns in series is compared to operation where the flow rate is decreased to compensate for a loss of enzyme activity for both zero‐and first‐order decay. The analyses indicated that columns in series resulted in better enzyme utilization but larger reactor volumes than parallel reactors with decreasing flow rate.