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Protease production by immobilized mycelia of Streptomyces fradiae
Author(s) -
Kokubu Toshinori,
Karube Isao,
Suzuki Shuichi
Publication year - 1981
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.260230104
Subject(s) - mycelium , protease , acrylamide , chemistry , streptomyces fradiae , yeast extract , food science , chromatography , polyacrylamide gel electrophoresis , biochemistry , enzyme , biology , streptomyces , fermentation , botany , organic chemistry , polymer , monomer , bacteria , actinomycetales , genetics
Abstract Streptomyces fradiae was immobilized in polyacrylamide gel prepared from 5% total acrylamide (90% acrylamide and 10% N , N ′‐methylenebisacrylamide). Production of protease by the immobilized mycelia was attempted in a batch system. A dilute medium containing 0.5% starch, 0.5% meat extract, and 0.05% yeast extract was employed. The reusability of the immobilized and washed mycelia was examined. The activity of protease production by washed mycelia was rapidly decreased with increasing use cycles. The activity of the immobilized mycelia increased gradually, and reached a maximum after ten use cycles. Then, the activity gradually decreased with increasing reaction cycles. This might be caused by destruction of the gels. On the other hand, the sterilization of the surface of the immobilized mycelia was effective for elongation of the lifetime. As a result, the half‐life of protease production by the sterilized immobilized mycelia was about 30 days. The rate of protease production by immobilized mycelia was 12,000 U/ml/hr. This value was four times higher than that by submerged culture.