Increased production of cellulase of  Trichoderma  sp. by pH cycling and temperature profiling | Zendy

Mukhopadhyay S. N. | Zendy; Malik R. K. | Zendy

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Increased production of cellulase of Trichoderma sp. by pH cycling and temperature profiling

Author(s) -

Mukhopadhyay S. N.,

Malik R. K.

Publication year - 1980

Publication title -

biotechnology and bioengineering

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.136

H-Index - 189

eISSN - 1097-0290

pISSN - 0006-3592

DOI - 10.1002/bit.260221104

Subject(s) - cellulase , liter , cellulose , trichoderma reesei , chemistry , cycling , chromatography , zoology , food science , biochemistry , biology , endocrinology , history , archaeology

Cultivation of Trichoderma reesei QM 9414 on 3% (w/v) cellulose medium (C/N ratio = 8.5) produced 4.5 IU/ml celulase 180 hr at a cell growth of 8.0 g/liter (0.266 g cell/g cellulose). It corresponded to an average cellulase productivity 25.0 IU/liter/hr (3.5 IU/g cell/hr). In the same medium 9.5 g/liter cell mass (0.316 g cell/g cellulose), 6.2 IU/ml cellulase, and 38.75 IU/liter/hr (4.0 IU/g cell/hr) cellulase productivity could be obtained using pH cycling condition during cultivation. Cell mass, cellulase yield, and productivity were further increased to 10.0 g/liter, 7.2 IU/ml, and 44.0 IU/liter/hr (4.5 IU/g cell/hr), respectively, by simultaneous pH cycling and temperature profiling strategy. Results are described.

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