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Batch‐ and continuous‐culture studies of a methane‐utilizing mixed culture
Author(s) -
Lamb S. C.,
Garver J. C.
Publication year - 1980
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.260221009
Subject(s) - methane , chemistry , biochemical engineering , organic chemistry , engineering
A methane‐utilizing mixed culture isolated from activated sludge by selective enrichment at 45°C was found to consist of three interacting species: a methaneutilizing bacterium, a citrate‐utilizing bacterium, and a methanol‐utilizing bacterium. All three species grew well at 45°C. Three different stable mixed cultures were reconstituted by various combinations of these pure cultures. The nutritional requirements and substrate ranges for each pure culture were determined. The nutritional requirements and substrate ranges for each pure culture were determined. The saturation constant for the methane‐utilizing bacterium on methane ( K CH 4) and for the methanol‐utilizing bacterium on methanol ( K CH 3 OH ) were 1.73 × 10 −6 M and 4.51 × 10 −7 M , respectively. The volumetric mass transfer coefficient for methane ( K L a ) was determined to be 65.6 hr −1 .