Premium
Batch and multistage continuous ethanol fermentation of cellulose hydrolysate and optimum design of fermentor by graphical analysis
Author(s) -
Tyagi R. D.,
Ghose T. K.
Publication year - 1980
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.260220910
Subject(s) - hydrolysate , fermentation , bagasse , chemistry , bioreactor , dilution , chromatography , continuous reactor , yeast , biomass (ecology) , ethanol fermentation , ethanol fuel , cellulose , ethanol , hydrolysis , pulp and paper industry , food science , biochemistry , microbiology and biotechnology , biology , organic chemistry , thermodynamics , agronomy , physics , catalysis , engineering
Batch and single‐flow four‐stage continuous ethanol fermentations of bagasse hydrolysate have been investigated at pH 4.0 and 30°C with a strain of the yeast Saccharomyces cerevisiae . The studies were carried out in the laboratory four‐stage cascade continuous stirred‐tank fermentors at varying feed glucose concentrations (10, 14, 18, and 22%). The range of dilution rates employed varied from 0.05 to 0.2 hr −1 . The hydrolysate was supplemented with a cheap nitrogen source (CNS), CaCl 2 ·H 2 O and MgSO 4 ·7H 2 O. A 2% (v/v) CNS concentration was found to be sufficient to avoid growth limitation at a glucose concentration of 116 g/liter. The conditions of continuous culture in a multistage system are predicted by a graphical method based on batch‐culture data. The results thus obtained are compared with those predicted by kinetic models and with the experimental results. The variations between the results obtained experimentally and those computed either by a kinetic model or by graphical analyses were found to be within the limits of experimental error. The solutions based on the concept of minimum residence time necessary to achieve the desired biomass or product concentrations are also discussed.