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Immobilization and properties of Leuconostoc mesenteroids dextransucrase
Author(s) -
Kaboli Hossein,
Reilly Peter J.
Publication year - 1980
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.260220513
Subject(s) - dextransucrase , leuconostoc mesenteroides , chemistry , ultrafiltration (renal) , size exclusion chromatography , chromatography , leuconostoc , yield (engineering) , biochemistry , lactic acid , bacteria , enzyme , fermentation , genetics , materials science , metallurgy , lactobacillus , biology
Dextransucrase from Leuconostoc mesenteroides (NRRL B‐512F) was purified by ultrafiltration and gel filtration chromatography in 54% yield. The specific activity of a heart cut was 58.6 U/mg; cumulative purification of that preparation was 247−fold. Of 13 carriers surveyed, only alkylamine porous silica gave immobilization efficiencies consistently above 15 %. Immobilization to silica changed the properties of dextransucrase relatively little, the optimum pH for activity remaining at 5.2, while that for stability decreased from pH 5.5−6 to pH 5.2. In short assays, highest activities of both soluble and immobilized dextransucrase occurred at 30°C. Activation energies below that temperature were 8.6 kcal/mol for the former form and 1.7 kcal/mol for the latter. Maximum stabilization of soluble dextransucrase was attained by 5m M Ca 2+ .