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Adsorption and recovery of cellulases during hydrolysis of newspaper
Author(s) -
Casta Maris,
Wilke Charles R.
Publication year - 1980
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.260220512
Subject(s) - cellulase , trichoderma viride , hydrolysate , hydrolysis , adsorption , chemistry , substrate (aquarium) , enzyme , chromatography , enzymatic hydrolysis , cellulose , trichoderma reesei , biochemistry , organic chemistry , food science , biology , ecology
The adsorption of cellulases from Trichoderma viride was studied during the hydrolysis of newspaper. By measuring individual enzyme activities it was found that in the early stage of hydrolysis enzyme components showing C x A; were adsorbed preferentially to those showing C 1 A; afterwards ths situation was inverted. Electrophoretic resolution of proteins in hydrolysates showed a continuous decrease of enzyme proteins in solution, and furthermore suggested that the enzymes once adsorbed remained immobilized on the substrate (even after extensive digestion). Experiments to recover the enzyme that had remained in solution after typical hydrolysis showed a potential saving of up to 40%.