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Staining method for determination of the penetration of immobilized enzyme into a porous support
Author(s) -
Carleysmith S. W.,
Eames M. B. L.,
Lilly M. D.
Publication year - 1980
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.260220505
Subject(s) - amberlite , penetration (warfare) , chromatography , staining , bovine serum albumin , chemistry , penicillin , enzyme , immobilized enzyme , biochemistry , antibiotics , biology , organic chemistry , mathematics , adsorption , operations research , genetics
The penetration of bovine serum albumin and penicillin acylase into Amberlite XAD7 beads was determined by staining split beads. The rates of penetration were measured and correlated with a theoretically derived equation.
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