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Yield regulation of lysine biosynthesis in Brevibacterium flavum
Author(s) -
Shvinka J.,
Viesturs U.,
Ruklisha M.
Publication year - 1980
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.260220413
Subject(s) - glyoxylate cycle , lysine , biosynthesis , biochemistry , carboxylation , metabolic pathway , chemistry , yield (engineering) , enzyme , metabolism , brevibacterium , pyruvate carboxylase , amino acid , biology , bacteria , microorganism , materials science , metallurgy , catalysis , genetics
A scheme for lysine biosynthesis using variants of the Brevibacterium flavum intermediary metabolite synthesis is discussed. The main precursor of lysine that we are concerned with here is oxalacetate, which can be synthesized through the TCA or glyoxylate cycles or by carboxylation of PEP. Material energy balances for the main pathways of lysine biosynthesis from glucose and acetate have been formulated. Energy consumption, in the from of ATP – P ATP (number of mol ATP consumed/1 mol lysine synthesized), was calculated for the main pathways of lysine biosynthesis. Theoretical conversion yields Y p max (g product/g substrate) were estimated. Experimental data were presented concerning the increase of Y p by means of metabolism regulation: (a) by TCA‐and glyoxylate‐cycle enzyme induction; (b) by maintaining PEP carboxylase activity; (c) by eliminating by‐product synthesis.