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Estimation of Trichoderma QM 9414 biomass and growth rate by indirect means
Author(s) -
Harima Takeshi,
Humphrey Arthur E.
Publication year - 1980
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.260220407
Subject(s) - chemostat , adenosine triphosphate , carbon dioxide , growth rate , biomass (ecology) , biochemistry , respiration , metabolism , biology , chemistry , food science , botany , ecology , bacteria , genetics , geometry , mathematics
Trichoderma QM 9414 was aerobically grown on glucose as the sole carbon and energy sources in a chemostat culture. The specific rates of glucose consumption ( Q G ), oxygen consumption ( Q O 2), and carbon dioxide production ( Q CO 2) at the steady state were measured to estimate the growth and maintenance requirements. From the results it was estimated that 2 mol adenosine triphosphate (ATP) were produced when1 mol NADH was oxidized through the respiratory chain of this microorganism. The true growth yield for ATP ( Y ATP ) and specific ATP consumption rate for maintenance ( Q ATP m) calculated with this value were 0.0106 g dry cell/mmol ATP and 5.2 mmol ATP/g dry cell/hr, respectively. Using the relationships between specific growth rate (μ) and ( Q CO 2) and between μ and Q G obtained from chemostat‐culture data, cell and glucose concentration histories were estimated from the carbon dioxide production rate during the batch culture. The estimated cell concentrations agreed with the experimentally measured values. Glucose concentration were slightly overestimated.