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Characteristics of hexadecane partition by the growth medium of Acinetobacter sp.
Author(s) -
Käppeli O.,
Finnerty W. R.
Publication year - 1980
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.260220303
Subject(s) - hexadecane , partition coefficient , solubility , chemistry , chromatography , penetration (warfare) , environmental chemistry , organic chemistry , operations research , engineering
The partition of n ‐hexadecane in the spent growth medium of Acinetobacter sp. HOI‐N was determined by measuring the increase in the relative aqueous solubility of 3 H‐hexadecane as compared to controls. The amount of hexadecane partitioned was proportional to the protein concentration. The specific solubility of hexadecane (nmol/mg protein) was analyzed by least‐squares fitting yielding an average slope of 0.6 with a standard deviation of 0.3, indicating either nonequilibrium of hexadecane or physical aggregation of protein. The amount of hexadecane partitioned was concentration dependent yielding optically clear microemulsions at hexadecane concentrations of less than 1.4m M and macroemulsions at hexadecane concentrations of 1.4m M or greater. Preliminary results indicated that hexadecane and partitioned by a lipoprotein complex.