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Dynamic aspects of radiorespirometry during the cell cycle of Candida utilis : Some observations in phased culture under carbon‐, nitrogen‐, and phosphorus‐limited growth
Author(s) -
Dawson Peter S. S.,
Steinhauer Lee P.
Publication year - 1980
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.260220111
Subject(s) - chemostat , citric acid cycle , cell cycle , biology , hexose , metabolism , biochemistry , cell growth , glycolysis , microbiology and biotechnology , cell , genetics , bacteria , enzyme
Many changes that occur in a cell during the cell cycle can be demonstrated in synchronous cultures and can reveal dimensions of cell metabolism not attainable by the study of balanced growth of asynchronous populations in batch cultures or the steady state in chemostat cultures. The release of 14 CO 2 from specifically labeled glucose by phased (continuously synchronized) cultures follows a characteristic pattern ( profile ) that depends upon the stage in the cell cycle and the period of labeling used. Successive profiles throughout a cycle showed differences that were altered under different nutrient‐limiting growth conditions. Profiles obtained with glucose‐1‐ 14 C, glucose‐2‐ 14 C, glucose‐3,4‐ 14 C, and glucose‐6‐ 14 C and phased cells of Candida utilis under N‐, P‐, and C‐limited growth demonstrated the variable character of the metabolic activity that occurred in the cells while contour changes within the profiles across the cycle indicated possible correlations with activities of the hexose monophosphate, Embden‐Meyerhof‐Parnas, and tricarboxylic acid cycle pathways during the cell cycle. The basis of these changes and their use as elementary parameters for study of problems of physiological changes in vivo are considered.