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Feedback control of a competitive mixed‐culture system
Author(s) -
Wilder Carl T.,
Cadman Theodore W.,
Hatch Randolph T.
Publication year - 1980
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.260220108
Subject(s) - corynebacterium glutamicum , constant (computer programming) , feedback control , dilution , control theory (sociology) , time constant , substrate (aquarium) , biological system , growth rate , control system , transient (computer programming) , chemistry , control (management) , mathematics , computer science , biology , thermodynamics , physics , ecology , biochemistry , control engineering , engineering , geometry , electrical engineering , operating system , artificial intelligence , gene , programming language
Abstract Three feedback strategies for the on‐line control of cell densities in a mixed‐culture system have been examined. A competitive mixed‐culture system of Candida utilis and Corynebacterium glutamicum grown on glucose as the limiting carbon source was modeled using Monod growth kinetics. First‐order time constants were added to simulate transient growth effects. Multivariable feedback control of cell densities by manipulation of substrate feed and dilution rate was investigated. Feedback strategies directed to minimizing control interactions were found to be superior to classical feedback. Transients in the growth‐rate response produced oscillations in cell density and required retuning of control constants. The relative time constants of the two species were important, with the largest oscillations resulting when the faster growing organism had the faster time constant.