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Concentration of baboon endogenous virus in large‐scale production by use of hollow‐fiber ultrafiltration technology
Author(s) -
Weiss S. A.
Publication year - 1980
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.260220103
Subject(s) - ultrafiltration (renal) , baboon , virus , endogeny , microcarrier , chemistry , biology , lactose , chromatography , virology , biochemistry , cell , endocrinology
A process is described in which the baboon endogenous virus (BaEV) is produced under optimum conditions in cell culture, and concentrated by hollow‐fiber ultrafiltration technology under conditions of large‐scale production. This system has advantages over conventional systems in that the flow rate is increased 2.5‐fold during concentration. Thermal inactivation of BaEV was retarded by the addition of lactose glutamate to the harvested tissue culture fluid. After concentration, at least 91% of the virus RNA‐directed DNA polymerase is recovered with a concomitant increase in infectious virus. Materials needed for modifying described systems may be obtained from commercial sources.