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Enzymatic lysis of yeast cell walls
Author(s) -
Knorr D.,
Shetty K. J.,
Kinsella J. E.
Publication year - 1979
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.260211109
Subject(s) - lysozyme , yeast , lysis , succinylation , enzyme , biochemistry , chemistry , cell wall , yield (engineering) , nucleic acid , cell disruption , chromatography , biology , amino acid , lysine , materials science , metallurgy
Lysis of yeast cell walls using zymolase and lysozyme was studied. During coupled zymolase–lysozyme treatment, nearly three times more reducing sugars were released from the yeast cells compared to controls. Enzyme treatment followed by extraction at pH 9 resulted in a yield of more than 80% of the total nitrogen of the yeast cell. Protein degradation occurred during enzyme treatment. The precipitation of proteins was significantly increased by succinylation after enzyme treatment. This also reduced the nucleic acid content of the yeast proteins to less than 2% and enhanced the extractability of nitrogenous material.