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Analysis of fermentation processes using flow microflourometry: Single‐parameter observations of batch bacterial growth
Author(s) -
FazelMadjlessi Jila,
Bailey J. E.
Publication year - 1979
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.260211108
Subject(s) - bacillus subtilis , fermentation , biological system , stationary phase , enumeration , bacterial growth , decomposition , metabolite , chemistry , batch processing , spore , biology , chromatography , bacteria , biochemistry , microbiology and biotechnology , mathematics , genetics , computer science , organic chemistry , combinatorics , programming language
The laser flow microfluorometer (FMF) can determine the amounts of certain components in single cells at sample rates of several thousand cells per second. This technique has been employed to characterize Bacillus subtilis populations in batch fermentations with different inocula. Protein and distributions obtained by FMF analyses at different times during the batch have been decomposed using an optimized fit of summed subpopulation distributions. The results of these decomposition calculations, some of which have been approximately confirmed by independent microscopic observations, indicate cells relative numbers of single rods, cell chains, spores, and swollen rounded cells change dramatically during the entire fermentation including the stationary phase. The dynamics of these subpopulations may be related to secondary metabolite production.