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Influence of substrate on the amino acid profile and macromolecular composition of a Brevibacterium species
Author(s) -
Miller Leslie T.,
Thayer D. W.
Publication year - 1979
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.260211107
Subject(s) - valine , amino acid , chemistry , substrate (aquarium) , biochemistry , brevibacterium , fumaric acid , cystine , threonine , food science , chromatography , biology , bacteria , microorganism , serine , enzyme , ecology , cysteine , genetics
Abstract Brevibacterium JM98A (ATCC 29895) was grown aerobically in carbon‐limited continuous culture on the following substrates: gluconic acid, galactose, fumaric acid, glutamic acid, and aspapagine. Both whole and trichloacetic acid(TCA)‐ex‐tracted cells were analyzed for their amino acid compositions. No significant variations of amino acid profile were induced by change of substrate. Only the valine content varied significantly with growth rate. Some significant variations were observed between whole and extracted cell samples, primarily in the levels of the essential amino acids threonine, cystine, and valine.