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Use of an enzyme thermistor in continuous measurements and enzyme reactor control
Author(s) -
Danielsson Bengt,
Mattiasson Bo,
Karlsson Roland,
Winqvist Fredrik
Publication year - 1979
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.260211005
Subject(s) - thermistor , glucose oxidase , immobilized enzyme , lactose , chemistry , chromatography , continuous reactor , substrate (aquarium) , continuous stirred tank reactor , enzyme , biochemistry , catalysis , biology , physics , ecology , quantum mechanics
Abstract The enzyme thermistor measures the heat produced by the action of an immobilized enzyme on a substrate present in the sample. Its application in analysis of discrete samples, e.g., in clinical chemistry, is well documented, but it has not been used so far for continuous measurements. We describe here the application of the enzyme thermistor for continuous monitoring and control of enzyme reactors. An enzyme thermistor filled with coimmobilized glucose oxidase and catalase was used to measure the amount of glucose in the outflow from a column reactor containging immobilized lactase acting on a lactose solution pumped through the reactor. The lactose conversion was kept on a constant level, irrespective of the actual enzymatic activity in the reactor, by regulating the flow through the reactor. The experiments were carried out with aqueous solutions of lactose as well as with whey from cow's milk.