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New nonsynthetic medium for Rhizobium culture production from wastes
Author(s) -
Gulati S. L.
Publication year - 1979
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.260210902
Subject(s) - trichoderma viride , mycelium , hyacinth , husk , fermentation , mannitol , biology , food science , yeast , yeast extract , botany , biochemistry , paleontology
A nonsynthetic medium was formulated for placement of mannitol fully by saccharified pea husk ( Pisum sativum L.) and water hyacinth ( Eichhoornia crassipes ) with Trichoderma viride QM 9414 and molasses. Yeast extract was Partially replaced by proteolysed pea husk, water hyacinth, and mycelium of T. viride QM 9414 by boiling 4 hr with 5% (v/v) HCl. The rhizobial growth was equal in both standard yeast extract mannitol (YEM) and formulated nonsynthetic media. However, barring Rhizobium phaseoli (urid) E‐6, the rhizobial counts in thenon‐synthetic medium were higher then the counts in YEM medium. In the fermentor, rhizobial growth was also almost equal to YEM medium. These results indicated that costly ingredients like mannitol and yeast extract can be replaced by hydrolysates of pea husk, water hyacinth, mycelium of T. viride , and molasses.