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Identification of metabolic model: Citrate production from glucose by Candida lipolytica
Author(s) -
Aiba Shuichi,
Matsuoka Masayoshi
Publication year - 1979
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.260210806
Subject(s) - chemostat , citric acid cycle , glyoxylate cycle , flux (metallurgy) , biochemistry , metabolic flux analysis , carbon dioxide , chemistry , carboxylation , metabolic pathway , metabolite , fructose , isocitrate dehydrogenase , metabolism , biology , organic chemistry , enzyme , catalysis , bacteria , genetics
The concept of mass balance was used to analyze the metabolic pathways of citrate production by Candida lipolytica from glucose. Specific rates of glucose consumption, citrate and isocitrate productions, carbon dioxide evolution, and cellular syntheses of protein and carbohydrate were observed in an NH 4 + ‐limited chemostat culture. These data permitted one to assess the carbon flux in vivo by solving simultaneous carbon balance equations with respect to intermediary metabolite pools in the steady State. Among the three models considered here, model I (which coordinates the pyruvate carboxylation with the tricarboxylic acid cycle, but disregards the glyoxylate cycle) was considered plausible because the carbon flux calculated so far was acceptable. On the other hand, models II and III (which overlook the pyruvate carboxylation and the 2‐oxoglutarate dehydrogenation, respectively) were found to be most unlikely because of the unusual flux assessed from these models.