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Secretion of cellulase and β‐glucosidase by Trichoderma viride ITCC‐1433 in submerged culture on different substrates
Author(s) -
Herr Dieter
Publication year - 1979
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.260210805
Subject(s) - trichoderma viride , cellulase , cellulose , substrate (aquarium) , chemistry , enzyme , biochemistry , inducer , food science , biology , ecology , gene
Trichoderma viride ITCC‐1433 produces high yields of cellulase and especially β‐glucosidase when grown in submerged culture on different carbon sources. Cellulase synthesis was strongly repressed in the presence of glucose and only a low constitutive activity of β‐glucosidase and carboxymethylcellulase, but no Avicelase, could be demonstrated when culturing T. viride on glucose. With carboxymethylcellulose (CMC) as a substrate the secretion of enzyme as well as growth depended on the degree of substitution, but in general CMC cannot be regarded either as a powerful inducer or as a carbon source. With insoluble cellulose, maximum enzyme production and activities were obtained using an alkali‐treated cellulose powder. On this substrate the excretion of soluble protein into the culture broth increased and the protein concentration corresponded to cellulolytic activities.