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Proteolytic denaturation and methods of improving the stability of glucose isomerase preparations
Author(s) -
Adler David,
Lim Henry,
Cottle David,
Emery Alden
Publication year - 1979
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.260210804
Subject(s) - denaturation (fissile materials) , proteolysis , protease , chemistry , glucose 6 phosphate isomerase , isomerase , biochemistry , chromatography , enzyme , nuclear chemistry
Evidence is provided in support of proteolytic denaturation of free and immobilized preparations of glucose isomerase from a Bacillus species. A number of methods to improve the stability with respect to proteolysis have been tested and their advantages as well as shortcomings are discussed. These methods include hollow‐fiber treatment, gel permeation, thermal treatment, and addition of protease inhibitors. The half‐life of the free and the cellulose acetate fiber‐entrapped preparations of glucose isomerase can be significantly improved. For example, the hollow‐fiber treatment can improve the half‐life by an order of magnitude.